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E. coli strains

E. coli stammer

General

Statens Serum Institut has a strain bank containing more than 62,000 E. coli strains, of which most are clinical isolates. The collection contains both wild types and reference strains.

Product description

The reference strains are primarily sold as freeze-dried ampoules, whilst most wild type strains are sold as agar slant in transport medium. Freeze-dried strains can be stored for many years in unopened ampoules at room temperature. Once the ampoule is opened, the strain must be used immediately. Strains sent in agar slant must be plated out within a week from receipt and resown, as strains cannot survive in transport agar for long. All strains are typed for the antigen they are sold under just before sale.

Use

The strains sent in agar slant can be sown directly out onto a plate, while the freeze-dried ampoules must be added to 1 mL bouillon, incubated overnight at 37°C, and the entire ampoule contents must be transferred onto a plate. The plate is incubated overnight at 37°C, and the strain can now be used for the intended purpose, or frozen slants can be made of it. E. coli strains that cannot be typed can be sent to Statens Serum Institut, the International Escherichia Centre (WHO), Building 37K, Room 125C, Artillerivej 5, DK-2300 Copenhagen S, Denmark, for further investigation.

Product range

When purchasing bacterial strains, we kindly ask you to fill out a Material Transfer agreement.

We primarily sell reference strains and have reference strains for all O, H, K and F antigens, various toxins and other E. coli virulence factors.
The strain bank also contains wild type strains representing a wide selection of the possible serotypes and virulence factors in various combinations. When ordering a wild type strain, it is important to specify which antigen/virulence combinations are required, as our strain bank, as mentioned, consists of more than 62,000 strains. For example, an order may appear as follows:

A) An O149:H18 strain. The rest of the antigens and virulence factors are unimportant.

or

B) Three strains that all contain the eae gene. Serotype irrelevant.

or

C) Six O26 strains with different H antigens. 

Please note concerning the OK O serotypes, that except for the OK O strains OK O26, OK O45, OK O55, OK O114 and OK O157, the other OK O serotypes are matched with the O strains with the corresponding number e.g. OK O86 antisera is matched with the O86 strain.

Article
number		 Product  Antigens
81832  Reference strain O1 O1:K1:H7 
81833  Reference strain O2 O2:K1:H4 
81834  Reference strain O3 O3:K2ab:H2 
81835  Reference strain O4 O4:K3:H5 
81838 Reference strain O5 O5:K4:H4
81839 Reference strain O6 O6:K2a:H1
81840 Reference strain 07 07:K1:H-
81841 Reference strain O8 O8:K8:H4
81842 Reference strain O9 O9:K9:H12
81843 Reference strain O10 O10:K5:H4
81844 Reference strain O11 O11:K10:H10
81845 Reference strain O12 O12:K5:H-
81846 Reference strain O13 O13:K11:H11
81847 Reference strain O14 O14:K7:H-
81848 Reference strain O15 O15:K14:H4
81849 Reference strain O16 O16:K1:H-
81850 Reference strain O17 O17:K16:H18
81851 Reference strain O18ab  O18ab:K-:H14
81854 Reference strain O18ac O18ac:K5:H7
81855 Reference strain O19ab O19ab:K-:H7
81856 Reference strain O20 O20:K17:H-
81857 Reference strain O21 O21:K20:H-
81858 Reference strain O22 O22:K13:H1
81859 Reference strain O23 O23:K18ab:H15
81860 Reference strain O24 O24:K+:H-
81861 Reference strain O25 O25:K19:H12
81862 Reference strain O26 O26:H-
81864 Reference strain O27 O27:K-:H-
81865 Reference strain O28ab O28ab:K-:H-
81866 Reference strain O28ac O28ac:H-
81867 Reference strain O29 O29:K-:H10
81868 Reference strain O30 O30:K-:H-
81869 Reference strain O32 O32:K-:H19
81870 Reference strain O33 O33:K-:H-
81871 Reference strain O34 O34:K-:H10
81872 Reference strain O35 O35:K-:H10
81873 Reference strain O36 O36:K-:H9
81874 Reference strain O37 O37:K-:H10
81876 Reference strain O38 O38:K-:H26
81877 Reference strain O39 O39:K-:H-
81878 Reference strain O40 O40:K-:H4
81879 Reference strain O41 O41:K-:H40
81880 Reference strain O42 O42:K-:H37
81881 Reference strain O43 O43:K-:H2
81883 Reference strain O44 O44:H18
81886 Reference strain O45 O45:K1:H10
82027 Reference strain O46 O46:K-:H16
82028 Reference strain O48 O48:K-:H-
82029 Reference strain O49 O49:K+:H12
82030 Reference strain O50 O50:K-:H4
82031 Reference strain O51 O51:K-:H24
82033 Reference strain O52 O52:K-:H10
82034 Reference strain O53 O53:K-:H3
82035 Reference strain O54 O54:K-:H2
82036 Reference strain O55 O55:H-
82037 Reference strain O56 O56:K+:H-
82038 Reference strain O57 O57:K-:H-
82040 Reference strain O58 O58:K-:H27
82041 Reference strain O59 O59:K-:H19
82042 Reference strain O60 O60:K-:H33
82045 Reference strain O61 O61:K-:H19
82046 Reference strain O62 O62:K-:H30
82047 Reference strain O63 O63:K-:H-
82048 Reference strain O64 O64:K-:H-
82049 Reference strain O65 O65:K-:H-
82051 Reference strain O66 O66:K-:H25
82052 Reference strain O68 O68:K-:H4
82053 Reference strain O69 O69:K-:H38
82054 Reference strain O70 O70:K-:H42
82055 Reference strain O71 O71:K-:H12
82056 Reference strain O73 O73:K-:H31
82057 Reference strain O74 O74:K-:H39
82059 Reference strain O75 O75:K95:H5
82060 Reference strain O76 O76:K-:H8
82062 Reference strain O77 O77:K96;H-
82063 Reference strain O78 O78:H-
82064 Reference strain O79 O79:K-:H40
82081 Reference strain O80 O80:K-:H26
82082 Reference strain O81 O81:K97:H-
82083 Reference strain O82 O82:K-:H-
82084 Reference strain O83 O83:K-:H31
82085 Reference strain O84 O84:K-:H21
82086 Reference strain O85 O85:K-:H1
82088 Reference strain O86 O86:K-:H-
82089 Reference strain O87 O87:K-:H12
82090 Reference strain O88 O88:K-:H25
82091 Reference strain O89 O89:K-:H16
82092 Reference strain O90 O90:K-:H-
82098 Reference strain O91 O91:K-:H-
82099 Reference strain O92 O92:K-:H33
86717  Reference strain O93  O93:-:H16 
82100 Reference strain O95 O95:K+:H33
82102 Reference strain O96 O96:K-:H19
82103 Reference strain O97 O97:K-:H-
82104 Reference strain O98 O98:K-:H8
82105 Reference strain O99 O99:K-:H33
82106 Reference strain O100 O100:K-:H2
82107 Reference strain O101 O101:K-:H33
82108 Reference strain O102 O102:K-:H40
82109 Reference strain O103 O103:K+:H8
82110 Reference strain O104 O104:K-:H12
82111 Reference strain O105 O105:K-:H8
82112 Reference strain O106 O106:K-:H33
82113 Reference strain O107 O107:K98:H27
82114 Reference strain O108 O108:K-:H10
82115 Reference strain O109 O109:K-:H19
82116 Reference strain O110 O110:K-:H39
82118 Reference strain O111 O111:H-
82119 Reference strain O112ab O112ab:H18
82120 Reference strain O112ac O112ac:H-
82121 Reference strain O113 O113:H21
82122 Reference strain O114 O114:H32
82123 Reference strain O115 O115:K-:H18
82124 Reference strain O116 O116:K+:H10
82126 Reference strain O117 O117:K98:H4
82127 Reference strain O118 O118:K-:H-
82128 Reference strain O119 O119:H27
82129 Reference strain O120 O120:K18a:H6
82130 Reference strain O121 O121:K-:H10
82131 Reference strain O123 O123:K-:H16
82132 Reference strain O124 O124:H30
82133 Reference strain O125ab O125ab:H19
82134 Reference strain O125ac O125ac:H6
82136 Reference strain O126 O126:H2
82137 Reference strain O127 O127a:H-
82138 Reference strain O128ab O128ab:H2
82139 Reference strain O128ac O128ac:K-:H12
82140 Reference strain O129 O129:K-:H11
82141 Reference strain O130 O130:K-:H9
82142 Reference strain O131 O131:K-:H26
82143 Reference strain O132 O132:K+:H28
82266 Reference strain O133 O133:K-:H29
82267 Reference strain O134 O134:K-:H35
82268 Reference strain O135 O135:K-:H-
82269 Reference strain O136 O136:H-
82270 Reference strain O137 O137:H41
82271 Reference strain O138 O138:H14
82272 Reference strain O139 O139:K12:H1
82273 Reference strain O140 O140:K-:H43
82274 Reference strain O141 O141:K-:H4;F4ab
82275 Reference strain O141ac O141ac:H4
82277 Reference strain O142 O142:H6
82278 Reference strain O143 O143:K-:H-
82279 Reference strain O144 O144:K-:H-
82280 Reference strain O145 O145:K-:H-
82281 Reference strain O146 O146:K-:H21
82282 Reference strain O147 O147:H19;F4ac
82283 Reference strain O148 O148:K-:H28
82284 Reference strain O149 O149:H10
82285 Reference strain O150 O150:K93:H6
82286 Reference strain O151 O151:K-:H10
82287 Reference strain O152 O152:K-:H-
82288 Reference strain O153 O153:K-:H7
82289 Reference strain O154 O154:K94:H4
82290 Reference strain O155 O155:K-:H9
82291 Reference strain O156 O156:K-:H47
82292 Reference strain O157 O157:H19;F4ac
82293 Reference strain O158 O158:K-:H23
82295 Reference strain O159 O159:K-:H20
82296 Reference strain O160 O160:K-:H34
82297 Reference strain O161 O161:K-:H54
82298 Reference strain O162 O162:K-:H10
82299 Reference strain O163 O163:K-:H19
82300 Reference strain O164 O164:K-:H-
82301 Reference strain O165 O165:K-:H-
81913 Reference strain O166 O166:K-:H4
81914 Reference strain O167 O167:K-:H5
81915 Reference strain O168 O168:K-:H16
81916 Reference strain O169 O169:K-:H8
81917 Reference strain O170 O170:K-:H1
81918 Reference strain O171 O171:K-:H2
81919 Reference strain O172 O172:K-:H-
81920 Reference strain O173 O173:K-:H-
81921 Reference strain O174 O174:K-:H27
81922 Reference strain O175 O175:K-:H28
81923 Reference strain O176 O176:H-
81924 Reference strain O177 O177:H25
81925 Reference strain O178 O178:H7
81926 Reference strain O179 O179:H8
81928 Reference strain O180 O180:H-
81929 Reference strain O181 O181:H49
81930 Reference strain O182 O182:K48:H25
81931 Reference strain O183 O183:H18
81932 Reference strain O184 O184:K-:H11
81933 Reference strain O185 O185:H28
81934 Reference strain O186 O186:K-:H-
81829  Reference strain O187  O187:K-:H52 
81935 Reference strain H1 O2:K2ab:H1
85337  Reference strain H2  O43:K-:H2 
85338  Reference strain H3  O53:K-:H3 
85339  Reference strain H4  O2:K1:H4 
81936 Reference strain H6 O2:K1:H6
85341  Reference strain H7  O1:K1:H7 
81937 Reference strain H8 O2:K-:H8
85342  Reference strain H10  O11:K10:H10 
85343  Reference strain H11  O13:K11:H11 
85344  Reference strain H12  O9:K9:H12 
85345  Reference strain H14  O18ab:K-:H14 
85346  Reference strain H15  O23:K18ab:H15
81939 Reference strain H17 O15:K97:H17
85347  Reference strain H18  O17:K16:H18 
81940 Reference strain H19 O9:K36:H19
81941 Reference strain H20 O8:K49:H20
81942 Reference strain H21 O8:K49:H21
81943 Reference strain H23 O45:Kne:H23
81944 Reference strain H24 O51:K12:H24
81945 Reference strain H25 O15:K16:H25
85348  Reference strain H26  O131:K-:H26 
81946 Reference strain H27 O15:Kne:H27
85349  Reference strain H28  O132:K+:H28 
85350  Reference strain H29  O133:K-:H29 
81947 Reference strain H30 O38:Kne:H30
81948 Reference strain H31 O3:K-:H31
85351  Reference strain H32  O114:H32 
81949 Reference strain H33 O11:Kne:H33
81950 Reference strain H34 O86:H34
85352  Reference strain H35  O134:K-:H35 
81951 Reference strain H36 O86:H36
85353  Reference strain H37  O42:K-:H37 
85354  Reference strain H38  O69:K-:H38 
85355  Reference strain H39  O74:K-:H39 
85356  Reference strain H40  O79:K-:H40 
85357  Reference strain H41  O137:H41 
85358  Reference strain H42  O70:K-:H42 
85359  Reference strain H43  O140:K-:H43 
81952 Reference strain H44 O3:K19:H44
81953 Reference strain H45 O52:Kne:H45
81954 Reference strain H46 O26:H46
81955 Reference strain H47 O86:Kne:H47
81956 Reference strain H48 O16:Kne:H48
81957 Reference strain H49 O6:K13:H49
81958 Reference strain H51 O8:K50:H51
81959 Reference strain H52 O11:Kne:H52
81961 Reference strain H53 O148a:Kne:H53
85360  Reference strain H54  O161:K-:H54 
81962 Reference strain H55 O75:Kne:H55
81963 Reference strain H56 O139:K-:H56
85361  Reference strain K1  O2:K1:H4 
85362  Reference strain K2ab  O3:K2ab:H2 
85363  Reference strain K3  O4:K3:H5 
85364  Reference strain K4  O5:K4:H4 
85365  Reference strain K5  O10:K5:H4 
81964 Reference strain K6 O4:K6:H5
81965 Reference strain K7 O7:K7:H4
85366  Reference strain K8  O8:K8:H4 
85367  Reference strain K9  O9:K9:H12 
85368  Reference strain K10  O11:K10:H10 
85369 Reference strain K11  O13:K11:H11 
81966 Reference strain K12 O4:K12:H-
81967 Reference strain K13 O6:K13:H1
85370  Reference strain K14  O15:K14:H4 
85371  Reference strain K16  O17:K16:H18 
85372  Reference strain K17  O20:K17:H- 
81968 Reference strain K18a O23:K18a:H15
85373  Reference strain K18ab  O23:K18ab:H15 
85374  Reference strain K19  O25:K19:H12 
85375  Reference strain K20  O21:K20:H- 
81970 Reference strain K23 O25:K23:H1
81971 Reference strain K24 O83:K24:H31
81972 Reference strain K26 O9:K26:H-
81973 Reference strain K27 O8:K27:H-
81974 Reference strain K28 O9:K28:H-
81975 Reference strain K29 O9:K29:H-
81976 Reference strain K30 O9:K30:H12
81977 Reference strain K31 O9:K31:H-
81978 Reference strain K32 O9:K32:H19
81979 Reference strain K33 O9:K33:H-
81980 Reference strain K34 O9:K34:H-
81981 Reference strain K35 O9:K35:H-
81982 Reference strain K36 O9:K36:H19
81983 Reference strain K37 O9:K37:H-
81984 Reference strain K38 O9:K38:H-
81985 Reference strain K39 O9:K39:H9
81986 Reference strain K40 O8:K40:H9
81987 Reference strain K41 O8:K41:H11
81988 Reference strain K42 O8:K42:H-
81989 Reference strain K43 08:K43:H11
81990 Reference strain K44 O8:K44:H-
81991 Reference strain K45 O8:K45:H9
81992 Reference strain K46 O8:K46:K30
81993 Reference strain K47 O8:K47:H2
81994 Reference strain K48 O8:K48:H9
81995 Reference strain K49 O8:K49:H21
81996 Reference strain K50 O8:K50:H-
81997 Reference strain K51 O1:K51:H-
81998 Reference strain K52 O4:K52:H-
81999 Reference strain K53 O6:K53:H-
82000 Reference strain K54 O6:K54:H10
82001 Reference strain K55 O9:K55:H-
82002 Reference strain K57 O9:K57:H32
82003 Reference strain K83 O20:K83:H26
82004 Reference strain K84 O20:K84:H26
82005 Reference strain K87 O8:K87:H19
82006 Reference strain K92 O73:K92:H34
85376  Reference strain K93  O150:K93:H6 
85377  Reference strain K94  O154:K94:H4 
85378  Reference strain K95  O75:K95:H5 
85379  Reference strain K96  O77:K96:H- 
85380  Reference strain K97  O81:K97:H- 
85381  Reference strain K98  O107:K98:H27 
82007 Reference strain K100 O75:K100:H5
82008 Reference strain K101 O20:K101:H-
82009 Reference strain K102 O8:K102:H-
82010 Reference strain K103 O101:K103:H-
85333  Reference strain F4ab  O141:K-:H4;F4ab
85334  Reference strain F4ac  O147:H19;F4ac
85335  Reference strain F6  O20:K101:H-;F6
87258  Reference strain OK O26   
87256  Reference strain OK O45   
87259  Reference strain OK O55   
87255  Reference strain OK O114   
87257  Reference strain OK O157   
82168 Reference strain D2103,
not patogen,
PCR negative control		 K12
82169 DEC PCR test strain D2164 vtx1, vtx2, eae
82170 DEC PCR test strain D3404 vtx1, eae
82171 DEC PCR test strain Fr 1368 ipaH
82172 DEC PCR test strain D3313 eltA
82173 DEC PCR test strain D2258 stp
82174 DEC PCR test strain D2259 sth
62688 Wild type strains Please specify
the requested
antigens

 

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Statens Serum Institut
SSI Diagnostica
2 Herredsvejen
3400 Hillerød
Denmark

Fax: +45 4829 9179
Tel: +45 4829 9178

(inquiries)
(ordering)
www.ssi.dk/ssidiagnostica

Statens Serum Institut

National Institute for Health Data and Disease Control

5 Artillerivej

DK 2300 Copenhagen S

T  +45 3268 3268