Inga Laursen and Børge Teisner: Process for producing immunoglobulins for intravenous administration and other immunoglobulin products. International Patent Application No. WO 99/64462, 1999, assigned by State Serum Institute. Granted as Patent No. US 6,281,336 B1, Aug. 28, 2001. Granted in EU 2005.
Inga Laursen: Mannan-binding lectin, a new plasma product, production process and clinical application. Downstream by Amersham Bioscience; PPB abstracts, 15-17, 2001.
Inga Laursen: Purification process for production of mannan-binding lectin and an MBL medicinal product. International Patent Application No. WO 99/64453, 1999, assigned by State Serum Institute. Granted as Patent No. US 6, US 6,429,192, 2002.
P. Garred, T. Pressler, S. Lanng, H.O. Madsen, C. Moser, I. Laursen, F. Balstrup, C. Koch, C. Koch: Mannose-binding lectin (MBL) substitution in an MBL deficient patient with severe cystic fibrosis. Pediatr. Pulmonol. 33, 201-207, 2002.
K. Takahashi, J. Gordon, H. Liu, K.N. Sastry, J.E. Epstein, M. Motwani, I. Laursen, S. Thiel, J.C. Jensenius, M. Carroll, R.A.B. Ezekowitz: Lack of mannose-binding lectin-A enhances survival in a mouse model of acute septic peritonitis. Microbes Infect. 4, 773-784, 2002.
Jørgensen, C.S., Laursen, I., Houen, G. Purification process for large scale production of Gc-globulin, the Gc.globulin produced hereby, a use of Gc-globulin and a Gc-globulin medicinal product. United States Patent application Publication. Pub. No.: US 2003/0036638 A1. Feb. 20, 2003.
O.J. Bergmann, M. Christiansen, I. Laursen, P. Bang, N.E. Hansen, J. Ellegaard, C. Koch, V. Andersen: Low levels of Mannose-binding lectin do not affect occurrence of severe infections or duration of fever in acute myeloid leukaemia during remission induction therapy. Eur. J. Haematol. 70, 91-97, 2003.
H. Locht, M. Christiansen, I. Laursen: Reactive arthritis and serum levels of mannose binding lectin – lack of association. Clin. Exp. Immunol. 131, 169-173, 2003.
I. Laursen, Mannan-binding lectin (MBL) production from human plasma. Biochem. Soc. Trans. 31 (4), 758-762, 2003.
H. Valdimarsson, T. Vikingsdottir, P. Bang, S. Saevarsdottir, J.E. Gudjonsson, O. Oskarsson, M. Christiansen, L. Blou, I. Laursen, C. Koch: Human plasma derived mannose-binding lectin (MBL): A phase I safety and pharmacokinetic study. Scand. J. Immunol. 59, 97-102, 2004.
A. Steinø, C. S. Jørgensen, I. Laursen, G. Houen. Interaction of C1q with thereceptor calreticulin requires a conformational change in C1q. Scandinavian Journal of Immunology 59, 485-495, 2004.
C.S. Jørgensen, M. Christiansen, B. Nørgard-Pedersen, E. Østergaard, F.V. Schiødt, I. Laursen, G. Houen. Gc globulin (vitamin D-binding protein) levels: an inhibition ELISA assay for determination of the total concentration of Gc globulin in plasma and serum. Scand. J. Clin. Lab. Invest. 64, 157-166, 2004.
L. Vinner, D. Therrien, E. Wee, I. Laursen, T. Hanke, S.L. Corbet, A. Fomsgaard. Immune response in rhesus macaques after mixed modality immunisations with DNA, recombinant adenovirus and recombinant gp120 from human immunodeficiency virus type 1. APMIS 114 (10), 690-99, 2006.
P.H. Jensen, I. Laursen, F. Matthiesen, P. Højrup. Posttranslational modifications in human MBL and human recombinant MBL. Biochem Biophys Acta. 1774 (3), 335-44, 2007.
M. Christiansen, C.S. Jorgensen, I. Laursen, D.Hirschberg, P.Hojrup, G.Houen. Protein chemical characterization of Gc globulin (vitamin D-binding protein) isoforms; Gc-1f, Gc-1s and Gc-2. Biochem Biophys Acta. 1774 (4), 481-92, 2007.
I. Laursen, G. Houen, P. Hojrup, N. Brouwer, .B. Krogsoe, L. Blou, P.R. Hansen. Second-generation nanofiltered plasma-derived mannan-binding lectin product: process and characteristics. Vox Sang. 92 (4), 338-50, 2007.
C.S. Jørgensen, F.V. Schiødt, B. Dahl, I. Laursen, G. Houen. Comparison of rocket and crossed immuno-electrophoresis for determination of the level of actin complexing of Gc globulin. Scand J Clin Lab Invest. 67 (7), 767-777, 2007.
P. Bang, I. Laursen, K. Thornberg, J. Schierbeck, B. Nielsen, H. Valdimarsson, C. Koch, M Christiansen. The pharmacokinetic profile of plasma-derived mannan-binding lectin in healthy adult volunteers and patients with Staphylococcus Aureus septicaemia. Scandinavian Journal of Infectious Diseases. 40, 44-48, 2008.
R. Pagh, K. Duus, I. Laursen, P.R. Hansen, J. Mangor, N. Thielens, G.J. Arlaud, L. Kongerslev, P. Højrup, G. Houen. The chaperone and potential mannan-binding lectin (MBL) co-receptor calreticulin interacts with MBL through the binding site for MBL-associated serine proteases. FEBS J; 275, 515-26, 2008.
C.B. Svendsen, T. Hummelshøj, L. Munthe-Fog, N. Milman, P. Garred, I. A. Laursen, M. Christiansen, K. A. Krogfelt. Ficolins and Mannose-Binding Lectin in Danish Patients with Sarcoidosis. Respir Med; 102(9), 1237-42, 2008.
Inga Laursen, Peter Højrup, Gunnar Houen, and Michael Christiansen. Characterization of the 1st SSI purified MBL standard. Clin Chim Acta, 395, 159-161, 2008.
M. Bisch, M. Christiansen, I. Laursen, A.M. Engel, P.E. Holstein, B. Jørgensen, L. Iversen, T. Karlsmark. Is there a connection between MBL-deficiency and chronic leg ulcers. Acta Derm Venereol; 89 (3), 307-8, 2009.
F.N.J. Frakking, N. Brouwer, M.D. van de Wetering, I. Kleine Budde, P.F. Strengers, A.D.R. Huitema, I. Laursen, G. Houen, H.N. Caron, K.M. Dolman, T.W. Kuijpers. Safety and pharmacokinetics of plasma-derived MBL Substitution in children with chemotherapy-induced neutropenia. Eur J Cancer; 45, 505-512, 2009.
Nannette Brouwer, Florine N.J. Frakking, Marianne D. van de Wetering, Michel van Houdt, Margreet Hart, Ilona Kleine Budde, Paul F.W. Strengers, Inga Laursen, Gunnar Houen, Dirk Roos, Jens C. Jensenius, Huib N. Caron, Koert M. Dolman, Taco W. Kuijpers. Mannose-Binding Lectin (MBL) substitution: recovery of opsonic function in vivo lags behind MBL serum levels. J Immunol 185 (5): 3495-504, 2009.
M. Bitsch*, I. Laursen*, A.M. Engel, M. Christiansen, S. Olesen Larsen, L. Iversen. P.E. Holstein, T. Karlsmark. Epidemiology of leg and foot ulcer patients and the relation to serum levels of Mannan-binding lectin (MBL). Acta Derm Venereol 89, 607-11, 2009.
Bjarne Orskov, Malene L. Borresen, Bo Feldt-Rasmussen, Ole Østergaard, Inga Laursen, Svend Strandgaard. Estimating glomerular filtration rate using the new CKD-EPI equation and other equations in patients with autosomal dominant polycystic kidney disease. Am J Nephrol. 31(1): 53-57, 2010.
Anja H, Simonsen, Sara Bech, Inga Laursen, Lisette Salvesen, Kristian Winge, Anne Nørremølle, Gunhild Waldemar, Lene Werdelin, Jørgen E. Nielsen, James N. McGuire, Lena E. Hjermind. Proteomic investigations of the ventriculo-lumbar gradient in human CSF. Journal of Neuroscience Methods doi: 10.1016/j.jneumeth.2010.06.017
Jørgensen CS, Levantino G, Houen G, Jacobsen S, Halberg P, Ullman S, Khamashta MA, Asmussen K, Oxholm P, Jørgensen MK, Wenrooij WJV, Wiik A. Determination of autoantibodies to annexin XI in systemic autoimmune diseases. Lupus 2000;9:515-20.
Højrup P, Roepstorff P, Houen G. Human placental calreticulin: characterization of domain structure and posttranslational modifications. Eur J Biochem. 2001;269:2558-65.
Houen G. Aminoalkyl affinity matrices. J Biochem Biophys Meth. 2001;49:189-97.
Wilfingseder D, Sørensen BK, Houen G, Schwelberger HG.
Complex formation of human placental diamine oxidase. Inflammation Res. 2002;51:S89-S90.
Sørensen BK, Højrup P, Østergård E, Jørgensen CS, Enghild J, Ryder LR, Houen G. Silver staining of proteins on electroblotting membranes and intensification of silver staining of proteins separated by polyacrylamide gel electrophoresis. Anal Biochem. 2002;304:33-41.
Houen G, Olsen DT, Hansen PR, Petersen KB, Bakholt V. Preparation of Bioconjugates by Solid-Phase Conjugation to Ion Exchange Matrix-Adsorbed Carrier Proteins. Bioconjugate Chem. 2003;14:75-9.
Jørgensen CS, Ryder LR, Steinø A, Højrup P, Hansen J, Beyer NH, Heegaard NHH, Houen G. Dimerisation and oligomerisation of the chaperone calreticulin. Eur J Biochem. 2003;270:4140-48.
Jørgensen CS, Laursen I, Houen G. Purification process for large scale production of Gc-globulin, the Gc.globulin produced hereby, a use of Gc-globulin and a Gc-globulin medicinal product. United States Patent application Publication. Pub. No.: US 2003/0036638 A1. Feb. 20, 2003.
Jørgensen CS, Christiansen M, Nørgaard-Pedersen B, Østergaard E, Schiødt FV, Laursen I, and Houen G. Gc globulin (vitamin D-binding protein) levels: an inhibition ELISA assay for determaination of the total concentration of Gc globulin in plasma and serum. Scand J Clin Lab Invest. 2004;64:157-66.
Steinø A, Jørgensen CS, Laursen I, Houen G. Interaction of C1q with the receptor calreticulin requires a conformational change in C1q. Scand J Immunol. 2004;59:485-95.
Jørgensen CS, Jagd M, Sørensen BK, McGuire J, Barkholt V, Højrup P, Houen G. Efficacy and compatibility with mass spectrometry of methods for elution of proteins from sodium dodecyl sulfate-polyacrylamide gels and polyvinyldifluoride membranes. Anal Biochem. 2004;330:87-97.
Jørgensen CS, Hansen KB, Jacobsen S, Ullman D, Mikkelsen TL, Weile B, Madsen MH, Heegaard NHH, Wiik A, Houen G. Absence of high affinity calreticulin autoantibodies in patients with systemic rheumatic diseases and celiac disease. Scand J Clin Lab Invest. 2005;65:403-12.
Jørgensen CS, Trandum C, Larsen N, Ryder LR, Gajhede M, Skov LK, Højrup P, Barkholt V, Houen G. Conformational stability of calreticulin. Prot Pept Lett. 2005;12: 687-693.
Jørgensen CS, Christiansen M, Laursen I, Krogsøe LB, Højrup P, Blou L, Houen G. Large scale purification and characterisation of non-glycosylated Gc globulin (vitamin D-binding protein) from plasma fraction IV. Biotechnol Appl Biochem. 2006;44:35-44.
Laursen I, Houen G, Højrup P, Brouwer N, Krogsøe LB, Blou L, Hansen PR. A 2nd generation nanofiltered plasma-derived mannan-binding lectin (MBL) product: process and characteristics. Vox Sang. 2007;92:338-50.
Christiansen M, Jørgensen CS, Laursen IA, Hirschberg D, Højrup P, Houen G. Protein chemical characterization of Gc globulin isoforms; Gc1f, Gc-1s and Gc-2. Biochim Biophys Acta 2007;1774:481-92.
Jørgensen CS, Schiødt FV, Dahl B, Laursen I, Houen G. Comparison of rocket and crossed immuno-electrophoresis assays for determination of the level of actin complexing of Gc globulin. Scand J Clin Lab Invest. 2007;67:767-77.
Beyer NH, Milthers J, Lauridsen AB, Houen G. Autoantibodies to the proteasome in monosymptomatic optic neuritis may predict progression to multiple sclerosis. Scand J Clin Lab Invest. 2007;67:696-706.
Sandhu N, Duus K, Jørgensen CS, Hansen PR, Bruun SW, Pedersen LØ, Højrup P, Houen G. Peptide Binding Specificity of the Chaperone Calreticulin. Biochim Biophys Acta 2007;1774:701-13.
Duus K, Pagh RT, Holmskov U, Højrup P, Skov S, Houen G. Interaction of calreticulin with CD40 ligand, TRAIL and Fas ligand. Scand J Immunol. 2007;66:501-7.
Nielsen T, Thaysen-Andersen M, Larsen N, Jørgensen FS, Houen G, Højrup P. Determination of protein conformation by isotopically labelled cross-linking and dedicated software: application to the chaperone calreticulin. Int J Mass Spectrom. 2007;268:217-26.
Duus K, Hansen PR, Houen G. Interaction of calreticulin with amyloid beta peptide 1-42. Prot Pept Lett. 2008;15:103-7.
Pagh RT, Duus K, Laursen I, Hansen PR, Mangor J, Thielens J, Arlaud GJ, Kongerslev L, Højrup P, Houen G. The chaperone and potential mannan-binding lectin (MBL) co-receptor calreticulin interacts with MBL through the binding site for MBL-associated serine proteases. FEBS J; 2008;275:515-26.
Pedersen JT, Ostergaard J, Houen G, Heegaard NH. Affinity capillary electrophoresis for identification and investigation of human Gc-globulin (vitamin D-binding protein) and its isoforms interacting with G-actin. Electrophoresis 2008;29:1723-33.
Toft KN, Larsen N, Jørgensen FS, Højrup P, Houen G, Vestergaard B. Small angle X-ray scattering study of calreticulin reveals conformational plasticity. Biochim Biophys Acta 2008;1784:1265-70.
Laursen I, Højrup P, Houen G, Christiansen M. Characterization of the 1st SSI purified MBL standard. Clin Chim Acta 2008;395:159-61.
Lacroix M, Dumestre-Pérard C, Houen G, Arlaud GJ, Thielens NM. Residue Lys57 in the collagen-like region of human L-ficolin and its counterpart Lys47 in H-ficolin play a key role in the interaction with the mannan binding lectin associated serine proteases and the collectin receptor calreticulin. J Immunol. 2009;182:456-65.
Frakking FNJ, Brouwer N, Wetering MD, Budde IK, Strengers PFW, Huitema AD, Laursen I, Houen G, Caron HN, Dolman KN, Kuijpers TW. Safety and pharmacokinetics of plasma-derived mannose-binding lectin (MBL) substitution in children with chemotherapy-induced neutropenia. Eur J Cancer 2009;45:505-12.
Brouwer N, Frakking FN, van de Wetering MD, van Houdt M, Hart M, Budde IK, Strengers PF, Laursen I, Houen G, Roos D, Jensenius JC, Caron HN, Dolman KM, Kuijpers TW. Mannose-binding lectin (MBL) substitution: recovery of opsonic function in vivo lags behind MBL serum levels. J Immunol. 2009;183:3496-504.
Duus K, Sandhu N, Jørgensen CS, Hansen PR, Steinø A, Thaysen-Andersen M, Højrup P, Houen G. Interaction of the Chaperone Calreticulin with Proteins and Peptides of Different Structural Classes. Prot Pept Lett. 2009;16:1414-23.
Sylvest L, Bendiksen CD, Houen G. Phosphatase inhibitors with anti-angiogenic effect in vitro. APMIS 2009;118:49-59.
Kjær B, Struve C, Friis T, Engel A-M, Beyer NH, Højrup P, Houen G. Synthesis and anti-angiogenic effect of conjugates between serum albumin and non-steroidal anti-inflammatory drugs. Prot Pept Lett. 2010;17:121-30.
Duus K, Hansen EW, Tacnet P, Frachet P, Arlaud GJ, Thielens NM, Houen G. Direct interaction between CD91 and C1q. FEBS J. 2010;277:3526-37.
Svendsen AJ, Hjelmborg JV, Wiik A, Houen G, Kyvik KO, Junker P. Autoantibodies in twins discordant for rheumatoid arthritis. Ann Rheum Dis. 2010 Aug 10
Ravnsborg T, Houen G, Højrup P. The glycosylation of myeloperoxidase. Biochim Biophys Acta. 2010 Jul 16
Pihl TH, Jørgensen CS, Santoni-Rugiu E, Leifsson PS, Hansen EW, Laursen I, Houen G. Safety Pharmacology, Toxicology and Pharmacokinetic Assessment of Human Gc Globulin (Vitamin D Binding Protein). Basic Clin Pharmacol Toxicol. 2010 Jun 17.
Michelow IC, Dong M, Mungall BA, Yantosca LM, Lear C, Ji X, Karpel M, Rootes CL, Brudner M, Houen G, Eisen DP, Kinane TB, Takahashi K, Stahl GL, Olinger GG, Spear GT, Ezekowitz RA, Schmidt EV. A novel L-ficolin/mannose-binding lectin chimeric molecule with enhanced activity against Ebola virus. J Biol Chem. 2010 Aug 6;285(32):24729-39.